view article

Figure 2
SDS–PAGE profile of a dissolved crystal of the MEDI545 Fab–rhIFN-α-2a complex under reducing and nonreducing conditions. The 4–20% gradient gel (BioRad, Hercules, California, USA) was stained with Bio-Safe Coomassie Stain (BioRad). It shows rhIFN-α-2a migrating at around 15 and 20 kDa under nonreducing and reducing conditions, respectively. This difference is likely to be attributable to the presence of two internal disulfide bonds, the reduction of which alters the compactness (and thus the migration) of the molecule. The same argument can be applied to the MEDI545 Fab fragment, which migrated slightly under its expected molecular weight of 50 kDa under nonreducing conditions. As expected, its heavy and light chains both migrated at 25 kDa under reducing conditions.

ISSN: 2053-230X
Follow Acta Cryst. F
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds