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Figure 2
Positive-ion MALDI–TOF mass spectra of N-linked glycans from glycoforms of CTLA-4ex expressed in CHO-K1 cells with 1.5 mM NB-DNJ (a), CHO Lec3.2.8.1 cells with 0.5 mM NB-DNJ (b), CHO-K1 cells with 10 µM kifunensine (c), CHO Lec3.2.8.1 cells (d) and CHO-K1 cells (e). A trace amount of putative Man3GlcNAc2 and Man4GlcNAc2 glycans was detected in the spectra of CHO Lec3.2.8.1 but was obscured by contaminating peaks (not shown). Symbols used for the structural formulae: diamonds, Gal; filled diamonds, GalNAc; filled squares, GlcNAc; circles, Man; stars, sialic acid; diamonds with dots in, Fuc. The linkage position is shown by the angle of the lines linking the sugar residues (vertical line, 2-link; forward slash, 3-link; horizontal line, 4-link; back slash, 6-link), whilst the anomericity is indicated by full lines for α-bonds and broken lines for β-bonds (Harvey et al., 2009BB20).

ISSN: 2053-230X
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