High-resolution structure of a new crystal form of BamA POTRA4–5 from Escherichia coli

The crystal structure of POTRA4–5 has been determined to 1.50 Å resolution with an R factor of 14.7% and an R_free of 18.9%.

Structures of the rat complement regulator CrrY

The structure of rat CrrY_1–4 determined in two distinct crystal forms shows a pronounced bend at the interface between domains 3 and 4.

Structure of the Drosophila melanogaster Rab6 GTPase at 1.4 Å resolution

The crystal structure of Drosphila melanogaster Rab6 bound to the non-hydrolysable GTP analogue GMPPNP has been determined at a resolution of 1.4 Å.

The structure of LpxD from Pseudomonas aeruginosa at 1.3 Å resolution

The crystal structure of the bacterial protein LpxD from P. aeruginosa was solved and refined at 1.3 Å resolution. The overall domain architecture and biological assembly are similar to those found in previously solved structures of LpxD from other species.

Structure of a multicopper oxidase from the hyperthermophilic archaeon Pyrobaculum aerophilum

The crystal structure of an extremely thermostable multicopper oxidase from a hyperthermophile was determined.

Crystallographic characterization of the DIX domain of the Wnt signalling positive regulator Ccd1

The DIX domain of Ccd1 was purified and crystallized.

Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19

Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27–401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resolution.

Cloning, purification, crystallization and preliminary X-ray analysis of the catalytic domain of human receptor-like protein tyrosine phosphatase γ in three different crystal forms

The design, expression and purification of receptor protein tyrosine phosphatase γ and two mutants and their crystallization in three different crystal forms are described.

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a novel plant-type ferredoxin/thioredoxin reductase-like protein from Methanosarcina acetivorans

A novel ferredoxin/thioredoxin reductase-like protein from M. acetivorans was heterologously expressed in E. coli, purified and then subjected to crystallization. Preliminary X-ray diffraction studies revealed that the crystal belonged to a primitive cubic space group, with unit-cell parameters a = b = c = 92.72 Å.

Expression, crystallization and preliminary X-ray diffraction studies of thermostable β-1,3-xylanase from Thermotoga neapolitana strain DSM 4359

Highly thermostable β-1,3-xylanase from T. neapolitana strain DSM 4359 was prepared and crystallized. X-ray diffraction data were collected to a resolution of 1.82 Å.

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the hyperthermophilic nucleotidyltransferase TTHA1015 from Thermus thermophilus HB8

A hyperthermophilic nucleotidyltransferase encoded by the TTHA1015 gene from T. thermophilus was crystallized. X-ray diffraction data were collected to 1.7 Å resolution.

Use of the α-mannosidase I inhibitor kifunensine allows the crystallization of apo CTLA-4 homodimer produced in long-term cultures of Chinese hamster ovary cells

The α-mannosidase I inhibitor kifunensine inhibited N-glycan processing in long-term cultures of Chinese hamster ovary cells, allowing deglycosylation and crystallization of the homodimeric extracellular region of the inhibitory glycoprotein receptor CTLA-4 (CD152).

Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus

The C-terminal domain of SARS coronavirus nonstructural protein 2 was cloned, overexpressed, purified and crystallized; the crystals diffracted to 2.5 Å resolution.

Crystallization and preliminary crystallographic analysis of the phosphotriesterase-like lactonase from Geobacillus kaustophilus

Recombinant GK1506 from the thermophilic bacterium Geobacillus kaustophilus has been expressed, purified and crystallized. A 2.6 Å resolution native data set was collected from a single flash-cooled crystal.

Cloning, overexpression, purification, crystallization, and preliminary X-ray studies of SP_0149, the substrate binding protein of an ABC transporter from Streptococcus pneumoniae

The substrate binding protein (SP_0149) of an ABC transporter from Streptococcus pneumoniae was molecularly cloned, overexpressed and purified. Diffraction quality crystals were grown using the hanging-drop vapour-diffusion technique.

Crystallization and preliminary X-ray crystallographic studies of the ice-binding protein from the Antarctic yeast Leucosporidium sp. AY30

The ice-binding protein from Leucosporidium sp. AY30 was cloned, expressed, purified and crystallized. A complete data set was collected to 1.5 Å resolution.

Crystallization and preliminary X-ray analysis of a putative sensor histidine kinase domain: the C-terminal domain of HksP4 from Aquifex aeolicus VF5

The putative sensor histidine kinase domain of the cytoplasmic protein HksP4 from the hyperthermophilic bacterium A. aeolicus VF5 was expressed, purified and crystallized by the sitting-drop vapour-diffusion method. Crystals were obtained in the presence of ATP and AMPPNP; they were found to belong to the same space group P2₁2₁2₁ and diffracted X-rays to 3.1 and 2.9 Å resolution, respectively.

Purification, crystallization and preliminary X-ray crystallographic analysis of the C-terminal cytoplasmic domain of FlhB from Salmonella typhimurium

The cytoplasmic domain of FlhB from S. typhimurium has been purified and crystallized.

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of MsDpo4: a Y-family DNA polymerase from Mycobacterium smegmatis

MsDpo4 from M. smegmatis, a member of the Y-family of DNA polymerases, was expressed and purified to homogeneity. Crystals of native MsDpo4 in the apo state were obtained and X-ray diffraction data were collected to a maximum resolution of 2.6 Å.

Cloning, expression, purification and preliminary X-ray diffraction studies of a putative Mycobacterium smegmatis thiolase

The crystallization of a putative SCP-x thiolase from M. smegmatis is described.

Crystallization and preliminary X-ray analysis of 4-di­phosphocytidyl-2-C-methyl-D-erythritol kinase (IspE) from Mycobacterium tuberculosis

The 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE) from M. tuberculosis H37Rv was overexpressed in E. coli, purified and crystallized. Diffraction data for the native enzyme were collected to 2.1 Å resolution.

Overexpression, crystallization, and preliminary X-ray crystallographic analysis of shikimate de­hydrogenase from Thermotoga maritima

Shikimate dehydrogenase from Thermotoga maritima has been overexpressed, crystallized, and its crystal structure has been determined. X-ray diffraction data have been collected to 1.45 Å.

Crystallization and preliminary X-ray diffraction analysis of membrane-bound respiratory [NiFe] hydrogenase from Hydrogenovibrio marinus

Membrane-bound respiratory [NiFe] hydrogenase was purified from H. marinus and crystallized. A diffraction data set was collected to 1.25 Å resolution.

Purification, crystallization and X-ray diffraction study of extracellular dermal glycoprotein from carrot and the inhibition complex that it forms with an endo-β-glucanase from Aspergillus aculeatus

Crystallization of extracellular dermal glycoprotein and the inhibition complex that it forms with endo-β-glucanase.

Purification, crystallization and preliminary X-ray characterization of the pentamodular arabinoxylanase CtXyl5A from Clostridium thermocellum

The large pentamodular C. thermocellum cellulosomal arabinoxylanase CtXyl5A (without the C-terminal dockerin) has been crystallized and data have been collected to a resolution of 2.64 Å.