Figure 1
Purification of rCwp1927–401. The first seven lanes contain material obtained using Tris-based IMAC buffers. Lane L, E. coli lysate. Lane FT, unbound material. Lanes E1–5, eluted fractions from early (E1) and late (E5) in the eluted peak. Lane E1 (PO4), early-eluted fraction from sodium phosphate (monobasic) based IMAC buffers. |