Figure 1
SDS–PAGE analysis of SARS-CoV nsp2C during purification. Proteins were analysed on 10% SDS–PAGE and stained with Coomassie Blue. Lane 1, molecular-weight markers (labelled in kDa). Lane 2, purified nsp2C after GST (glutathione S-transferase) affinity column chromatography. The molecular weight of GST-nsp2C is 84 kDa, as indicated by a black arrow. Lane 3, purified nsp2C with approximately 90% purity after Resource Q ion-exchange column chromatography. The molecular weight of nsp2C is 58 kDa, as indicated by a black arrow. |