 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](tb5063fig1mag.jpg)
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Figure 1
(a) 15% SDS–PAGE stained with Coomassie Brilliant Blue. Lanes 1 and 2 contain molecular-weight marker (labelled in kDa) and purified OsRac1-Q68L, respectively. (b) Elution profile of gel filtration of OsRac1-Q68L. The inset shows the calibration curve. The open rectangles correspond to the following marker proteins: A, thyroglobulin, 670.0 kDa; B, γ-globulin, 158.0 kDa; C, ovalbumin, 44.0 kDa; D, myoglobin, 17.0 kDa; E, vitamin B12, 1.4 kDa. The cross represents the peak-top position of OsRac1, assuming the molecular weight of the monomeric form containing GMPPNP and Mg2+ ion. MALDI–TOF MS analysis gave a single peak at 19 905.8 Da corresponding to the calculated molecular mass of OsRac1-Q68L (19 963.9 Da). |
![[Figure 1]](tb5063fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
