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Figure 1
SDS–PAGE analysis. The black box around the single band at approximately 26 kDa identifies the purified protein. The chimera L-BDH protein was separated using 12% SDS–PAGE and stained with Coomassie Brilliant Blue. Lane A, 16 µl cell culture; lane B, cell-free extract; lane C, ammonium sulfate fractionation; lane D, HiTrap Phenyl HP; lane E, HiTrap Q HP; lane F, Superdex 200 pg; lane M, molecular-weight marker (labelled in kDa). 10 µg protein was applied to each lane except for lane A.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 70| Part 4| March 2014| Pages 461-463
doi:10.1107/S2053230X13032755
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