Mechanisms, kinetics, impurities and defects: consequences in macromolecular crystallization

New imaging techniques, particularly AFM, permitted the elucidation of the mechanisms for protein and virus crystal growth. They have also allowed direct visualization of crystal defect structure and the consequences of impurity incorporation.

The structures of the CutA1 proteins from Thermus thermophilus and Pyrococcus horikoshii: characterization of metal-binding sites and metal-induced assembly

The crystal structures of CutA1 from T. thermophilus HB8 with and without bound Na⁺ and CutA1 from P. horikoshii OT3 in complex with Na⁺ have been determined in order to understand metal binding and metal-driven assembly features of the proteins.

1.55 Å resolution X-ray crystal structure of Rv3902c from Mycobacterium tuberculosis

The 1.55 Å resolution X-ray crystal structure of Rv3902c from M. tuberculosis reveals a novel fold.

1.15 Å resolution structure of the proteasome-assembly chaperone Nas2 PDZ domain

The proteasome-assembly chaperone Nas2 binds to the proteasome subunit Rpt5 using its PDZ domain. The structure of the Nas2 PDZ domain has been determined.

Structure of 4-pyridoxolactonase from Mesorhizobium loti

The structure of 4-pyridoxolactonase from M. loti was determined.

Comparison of four different crystal forms of the Mycobacterium tuberculosis ESX-1 secreted protein regulator EspR

Structural comparison of four crystal forms of Mycobacterium tuberculosis EspR protein.

Structure of the kinase domain of Gilgamesh from Drosophila melanogaster

The structure of the kinase domain of Gilgamesh from Drosophila melanogaster was determined at 2.85 Å resolution.

Structure of human nucleosome containing the testis-specific histone variant TSH2B

The crystal structure of human nucleosome containing the testis-specific TSH2B variant has been determined. The TSH2B Ser85 residue does not interact with H4 in the nucleosome, and induces a local structural difference between TSH2B and H2B in nucleosomes.

Structural characterization of a novel autonomous cohesin from Ruminococcus flavefaciens

The crystal structure of a standalone cohesin protein from the rumen bacterium R. flavefaciens has been determined at 2.44 Å resolution, revealing an elaborate set of secondary-structural elements.

Structure of the Reston ebolavirus VP30 C-terminal domain

The crystal structure of the Reston ebolavirus VP30 C-terminal domain shows a rotated interface in comparison to the previous structure of the Zaire ebolavirus VP30 C-terminal domain.

Crystallization and preliminary X-ray diffraction analysis of domain-chimeric L-(2S,3S)-butanediol de­hydrogenase

Crystallization of a domain-chimeric L-2,3-butanediol dehydrogenase (L-BDH) designed to possess both the S-configuration specificity of L-BDH and the stability of meso-BDH.

Crystallization and preliminary X-ray crystallographic analysis of α-glucosidase HaG from Halomonas sp. strain H11

The recombinant α-glucosidase HaG from Halomonas sp. Strain H11 was overexpressed, purified and crystallized to 2.15 Å, and its structure has been determined by molecular replacement.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of a mammalian type 10 adenylyl cyclase

Among the mammalian adenylyl cyclases, the soluble type 10 cyclase is unique in its activation by bicarbonate and calcium and its lack of a transmembrane region. Here, the recombinant production and crystallization of the catalytic core of the human type 10 adenylyl cyclase are described.

Crystallization and preliminary neutron diffraction experiment of human farnesyl pyrophosphate synthase complexed with risedronate

Neutron diffraction studies of human farnesyl pyrophosphate synthase are reported.

Crystallization and preliminary X-ray analysis of a novel type of lipolytic hydrolase from Bacillus licheniformis

The hydrolase BL28 from B. licheniformis was crystallized in the tetragonal space group P4₁2₁2 and diffraction data were collected to 1.67 Å resolution.

Preliminary crystallographic analysis of a double mutant of the acetyl xylo-oligosaccharide esterase Axe2 in its dimeric form

A rational double mutation in the serine acetyl xylo-oligosaccharide esterase from G. stearothermophilus T6 (Axe2) converts the enzyme from an octameric form to a dimeric form. The dimeric double mutant (Axe2-Y184F-W190P) has been crystallized in the orthorhombic space group P2₁2₁2₁ and a complete X-ray diffraction data set has been measured for these crystals at 2.3 Å resolution for use in a full three-dimensional structural analysis of this form of the protein.

Purification, crystallization and X-ray crystallographic studies on a putative methyltransferase, YtqB, from Bacillus subtilis

A putative methyltransferase, YtqB, from B. subtilis was overexpressed, purified and crystallized. X-ray diffraction data were collected from a YtqB crystal to 1.68 Å resolution.

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of (S)-3-hy­droxy­butyryl-CoA de­hydrogenase from Clostridium butyricum

3-Hydroxybutyryl-CoA dehydrogenase from C. butyricum, an enzyme involved in n-butanol production, has been crystallized and X-ray diffraction data from the crystal have been collected and analyzed.

Cloning, crystallization and preliminary X-ray diffraction analysis of an intact DNA methyltransferase of a type I restriction–modification enzyme from Vibrio vulnificus

The single-chain methyltransferase of a type I restriction–modification system was expressed and crystallized, and diffraction data were collected to a resolution of 2.31 Å.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of the ectoine hydroxylase from Sphingopyxis alaskensis

The ectoine hydroxylase from S. alaskensis was crystallized, yielding crystals diffracting to 2.1 Å resolution. The addition of iron prior to crystallization led to a different type of crystal, which may represent the iron-bound form of the enzyme.

Purification, crystallization and preliminary X-ray diffraction analysis of ArsH from Synechocystis sp. strain PCC 6803

ArsH from Synechocystis sp. strain PCC 6803 was overproduced, purified and crystallized. Diffraction data were processed to a resolution of 1.6 Å. The crystals belonged to the tetragonal space group I4₁22, with unit-cell parameters a = b = 127.94, c = 65.86 Å.

Crystallization and preliminary X-ray crystallographic analysis of importin-α from Neurospora crassa

The nuclear import receptor importin-α from the filamentous fungus N. crassa has been crystallized in the presence of a classical NLS peptide. An X-ray diffraction data set was collected and a molecular-replacement solution was obtained.

Expression, crystallization and preliminary X-ray crystallographic analysis of D-alanine-D-alanine ligase from OXA-23-producing Acinetobacter baumannii K0420859

OXA-23-producing A. baumannii K0420859 can resist the action of carbapenem, and causes bacteraemia, pneumonia, other respiratory-tract and urinary-tract infections in humans. As a novel target for an antibacterial drug against A. baumannii OXA-23, D-alanine-D-alanine ligase from A. baumannii was purified and crystallized, and a primary X-ray crystallographic analysis was performed.

Preliminary crystallographic analysis of the ankyrin-repeat domain of Arabidopsis thaliana AKT1: identification of the domain boundaries for protein crystallization

The construct optimization, purification, crystallization and preliminary crystallographic analysis of the ankyrin-repeat domain of Arabidopsis thaliana potassium transporter AKT1 is reported.

Crystallization and preliminary X-ray crystallographic studies of transglutaminase 2 in complex with Ca²⁺

Human transglutaminase 2 (TG2) in complex with Ca²⁺ was crystallized and the crystals were found to belong to the orthorhombic space group C222₁, with unit-cell parameters a = 133.08, b = 216.30, c = 166.26 Å. The crystals were obtained at 20°C and diffracted to a resolution of 3.4 Å.

Recombinant production, purification and crystallization of the Toxoplasma gondii coronin WD40 domain

The T. gondii coronin WD40 domain with the conserved region was recombinantly produced, purified and crystallized. A diffraction data set was collected to 1.65 Å resolution.

Crystallization and preliminary crystallographic analysis of manganese lipoxygenase

Manganese lipoxygenase of Geaumannomyces graminis was crystallized and X-ray diffraction data were collected to a resolution of 2.6 Å.

Successful data recovery from oscillation photographs containing strong polycrystalline diffraction rings from an unknown small-molecule contaminant: preliminary structure solution of Salmonella typhimurium pyridoxal kinase (PdxK)

Pyridoxal kinase (PdxK) from S. typhimurium was cloned, purified and crystallized. Successful structure solution was accomplished in spite of strong polycrystalline noise in the oscillation images.

Frq2 from Drosophila melanogaster: cloning, expression, purification, crystallization and preliminary X-ray analysis

The calcium-binding protein Frq2 from D. melanogaster has been crystallized and X-ray diffraction data from two different crystal forms have been collected.

Crystallization and preliminary X-ray crystallographic analysis of PBPD2 from Listeria monocytogenes

The crystallization of PBPD2 from L. monocytogenes is described.

Notes for authors