Figure 5
Examples of UV-imaging outcomes with the associated bright-field (visible) image: (a) the good, protein crystals fluorescing strongly (proteinase K) also easily identified visually, (b) the bad, salt crystals (calcium sulfate) with adsorbed protein, (c) the ugly, noncrystal information showing phase separation (also a potential lead condition for optimization) in a myoglobin-containing trial (myoglobin is excluded from one phase) and (d) the beautiful, an example of the identification of protein crystals that may have been easily missed visually. In each case a 100 µm scale bar is visible in the bottom left corner. |