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Figure 6
Despeckling (median filtering) applied by default to UV images can remove the signal from small protein crystals. During the process of median filtering, a pixel's value is replaced by the median value of the pixels around it. This image was taken at a magnification such that one pixel is approximately 1 µm, so that the very thin needles are approximately 1–2 µm wide and thus are removed during the median filtering step.

ISSN: 2053-230X
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