Figure 8
Examples of nanocrystals identified from granular aggregates using negative-stain TEM. For this study, a variety of challenging samples from membrane proteins to multi-protein complexes were used. Samples include three membrane proteins, CD3Delta, thermo-stabilized parathyroid hormone receptor 1 (tPTHR1) and the full-length influenza virus hemagglutinin protein (H5); a soluble protein, trans-acting acyl transferase from disorazole synthase (E. coli); and four multi-protein complexes, the five-component core of the human transcription factor TFIIH, the yeast heterodimer elongation complex Spt4/5, RNA polymerase II and a complex between RNA-Pol II, the transcription factor TFIIB, Spt4/5 and a 53-mer nucleic acid scaffold. Taken from Stevenson et al. (2014) with permission. |