Protein crystallization with microseed matrix screening: application to human germline antibody Fabs

Obmolova, G.; Malia, T.J.; Teplyakov, A.; Sweet, R.W.; Gilliland, G.L.

doi:10.1107/S2053230X14012552

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 5
Crystallization of Fab 5-51/A27. (a) Initial screening hit in 2.0 M AmSO4, 5% PEG 400, 0.1 M Tris pH 8.5. (b) Crystals in 2.0 M AmSO4, 0.1 M CHES pH 9.5 obtained by MMS. (c) Crystal in 1.0 M AmSO4, 0.1 M CHES pH 9.5 from refinement screening with diluted seeds.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 8| July 2014| Pages 1107-1115

doi:10.1107/S2053230X14012552

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