Figure 9
Crystallization of Fab 5-51/O12. (a)–(e) MMS results in 2.0 M AmSO4, 0.1 M CHES pH 9.5 with the following crystallization seeds: (a) AmSO4 + PEG mixture, (b) AmSO4 mixture, (c) AmSO4 mixture without Fab 5-51/A27, (d) only Fab 5-51/A27, (e) the same as (d) but with seeds diluted tenfold. (f) Crystals from refinement screening with the Fab 5-51/A27 seeds in 1.8 M AmSO4, 5% dioxane, 0.1 M CHES pH 9.5. |