 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](no5052fig1mag.jpg)
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Figure 1
SDS–PAGE of purification of the recombinant LjD-LDH protein. Lane 1, molecular-weight markers (labelled in kDa). Lanes 2–8 show the purification procedure of LjD-LDH using Ni–NTA chromatography. Lane 2, whole cell extract; lanes 3 and 4, supernatant and pellet fraction after centrifugation of whole cell extract, respectively; lane 5, flowthrough from the Ni–NTA column; lanes 6 and 7, washes with 5 and 10 mM imidazole, respectively; lane 8, elution with 300 mM imidazole. The 6×His LjD-LDH protein is indicated on the right side of the gel. |
![[Figure 1]](no5052fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
