Crystallization and preliminary X-ray diffraction analysis of YidC, a membrane-protein chaperone and insertase from Bacillus halodurans

Kumazaki, K.; Tsukazaki, T.; Nishizawa, T.; Tanaka, Y.; Kato, H.E.; Nakada-Nakura, Y.; Hirata, K.; Mori, Y.; Suga, H.; Dohmae, N.; Ishitani, R.; Nureki, O.

doi:10.1107/S2053230X14012540

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 1
Preparation of YidC_27–266 and cysteine mutants of YidC_27–266. (a) Construction of YidC_27–266. The amino-acid sequences of the N- and C-terminal regions of YidC are shown. A His₈ tag followed by the Tobacco etch virus (TEV) protease cleavage site were introduced at the 26th position. The C-terminal 14 residues were removed. (b) Methylmercury chloride labelling of wild-type YidC_27–266 and cysteine mutants of YidC_27–266. The tetramethylrhodamine-5-maleimide (TMRM) signals (top) and the gel stained with SimplyBlue SafeStain (bottom).

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 70| Part 8| July 2014| Pages 1056-1060

doi:10.1107/S2053230X14012540

Follow Acta Cryst. F

Search term		doi		Advanced search
Author		volume	page