Figure 1
Structure of RpfC compared with RpfB and RpfE. (a) RpfC asymmetric unit and cell edges looking at the ac plane. The noncrystallographic translation of (0.554, 0.0, 0.109) can be seen. (b) Superposition of RpfB [green; PDB entry 4kpm
chain A with tri-N-acetylglucosamine (NAG)3 and benzamidine], RpfC (light blue; PDB entry 4ow1
chain A with ethylene glycol) and RpfE (tan; PDB entry 4cge
chain A) with the small insertion at the bottom left of RpfB. (c) Comparison of RpfB [PDB entry 4kpm
; chain A, ice blue; chain B, gold; (NAG)3 and benzamidine in green ball-and-stick representation] with RpfC (PDB entry 4ow1
; chain A, ice blue; chain T, cyan; ethylene glycol, light blue) and lysozyme [PDB entry 1lzs
; chain A, red; (NAG)4 and (NAG)2 shown with fat red bonds]. This shows the conservation of a crystallographic interface between RpfB and RpfC and the overlap of the ethylene glycol and benzamidine sites. All images were produced with CCP4mg (McNicholas et al., 2011). |