 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](pg5052fig1mag.jpg)
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Figure 1
Documentation of the purification procedure. (a) SDS–PAGE separations of samples representing whole cell extracts (WCE), the soluble fraction after lysate clearance by ultracentrifugation and fractions of elution from immobilized metal-chelating chromatography. Lane M contains molecular-weight marker (labelled in kDa). (b) After pooling and concentration, the sample was further separated by size-exclusion chromatography on a Superose 6 10/300 column. The elution profiles of globular markers of the indicated molecular mass are shown. The elution profile and corresponding protein content are shown. The lanes marked L contain two concentrations of sample loaded onto the column. Lane M contains molecular-weight marker (labelled in kDa). |
![[Figure 1]](pg5052fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
