Large-volume protein crystal growth for neutron macromolecular crystallography

Growing large-volume crystals are necessary and important for neutron macromolecular crystallography. Experimental strategies, techniques and theoretical considerations for protein crystal growth are reviewed.

The X-ray structure of human P-cadherin EC1-EC2 in a closed conformation provides insight into the type I cadherin dimerization pathway

The crystal structure of human P-cadherin EC1-EC2 in a closed conformation is reported at 1.62 Å resolution, showing a double conformation of Trp2 in the binding pocket and a novel monomeric packing arrangement.

Structural basis of the substrate specificity of the FPOD/FAOD family revealed by fructosyl peptide oxidase from Eupenicillium terrenum

The crystal structure of fructosyl peptide oxidase from E. terrenum was determined at 1.9 Å resolution. This is the first structure of a group I FPOD that prefers α-fructosyl peptide substrates.

Crystallization and preliminary X-ray crystallographic analysis of the sclerostin-neutralizing Fab AbD09097

A neutralizing Fab antibody fragment directed against the Wnt antagonist sclerostin was crystallized and its structure was determined by molecular replacement.

Crystallization and preliminary X-ray diffraction analysis of the interaction of Aeromonas hydrophila MtaN-1 with S-adenosylhomocysteine

In this study, MtaN-1 from A. hydrophila was successfully expressed and purified, and crystals in complex with the substrate SAH were obtained and diffracted to a resolution of 1.4 Å.

Crystallization and preliminary X-ray diffraction analysis of an endo-1,4-β-D-glucanase from Aspergillus aculeatus F-50

A GH12 endoglucanase from A. aculeatus F-50 was expressed in P. pastoris, crystallized and diffracted to a resolution of 1.6 Å.

Purification, crystallization and preliminary X-ray analysis of the periplasmic haem-binding protein HutB from Vibrio cholerae

HutB from V. cholerae has been cloned, overexpressed, purified and crystallized in a monomeric form. Crystals belonging to space group P4₃2₁2 produced diffraction data to 2.4 Å resolution.

Crystallization of mutants of Turnip yellow mosaic virus protease/ubiquitin hydrolase designed to prevent protease self-recognition

Mutants of Turnip yellow mosaic virus protease/ubiquitin hydrolase designed to prevent self-recognition were produced and crystallized as monomers.

Cloning, expression, crystallization and preliminary X-ray studies of a superfolder GFP fusion of cyanobacterial Psb32

Psb32 from T. elongatus was expressed using a novel vector library for the generation of superfolder fluorescent fusion proteins. X-ray diffraction data were collected to a resolution of 2.3 Å from a crystal belonging to space group P6₁22.

Crystallization and preliminary X-ray characterization of the eukaryotic replication terminator Reb1–Ter DNA complex

This crystallization and data collection are reported for a complex of Reb1 bound to Ter3 DNA.

Structure of the omalizumab Fab

The therapeutic antibody omalizumab inhibits the binding of IgE to its receptors. Two crystal structures of the omalizumab Fab fragment have been determined at 1.9 and 3.0 Å resolution.

Expression, purification, crystallization and preliminary crystallographic analysis of a GH20 β-N-acetylglucosaminidase from the marine bacterium Vibrio harveyi

V. harveyi β-N-acetylglucosaminidase (VhGlcNAcase) is a glycoside hydrolase that degrades chitooligosacharides, yielding N-acetylglucosamine as the final product. Single crystals of wild-type and D437A-mutant VhGlcNAcase were grown and diffracted to resolutions of 2.37 and 2.60 Å, respectively.

Purification, crystallization and preliminary X-ray crystallographic analysis of the phosphatase domain (PA3346PD) of the response regulator PA3346 from Pseudomonas aeruginosa PAO1

The phosphatase domain PA3346PD of the response regulator PA3346 from P. aeruginosa has been overexpressed, purified and crystallized by the sitting-drop vapour-diffusion method.

Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod–Zwilch–ZW10 (RZZ) complex

The 800 kDa complex of the human Rod, Zwilch and ZW10 proteins (the RZZ complex) was reconstituted in insect cells, purified, crystallized and subjected to preliminary X-ray diffraction analysis.

Structure of the ABL2/ARG kinase in complex with dasatinib

This article reports the co-crystal structure of the mouse ARG catalytic domain with dasatinib, a tyrosine kinase inhibitor used for the treatment of leukemia.

Structure of di­hydrodipicolinate synthase from the commensal bacterium Bacteroides thetaiotaomicron at 2.1 Å resolution

The structure of dihydrodipicolinate synthase from B. thetaiotaomicron has been determined. The overall structure is described and compared with related structures.

Analytical ultracentrifugation and preliminary X-ray studies of the chloroplast envelope quinone oxidoreductase homologue from Arabidopsis thaliana

Analytical ultracentrifugation studies and crystallization experiments have been performed on the chloroplast envelope quinone oxydoreductase homologue (ceQORH). Diffracting crystals of ceQORH have been obtained and X-ray diffraction data were collected at 2.34 Å resolution.

Crystallization and preliminary X-ray analysis of four cysteine proteases from Ficus carica latex

Four ficin (iso)forms from F. carica latex were isolated and crystallized. For each form, at least one crystal form was obtained that diffracted to a resolution of at least 1.60 Å.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AerF from Microcystis aeruginosa, a putative reductase participating in aeruginosin biosynthesis

The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AerF from M. aeruginosa, which is a putative reductase that participates in the biosynthetic pathway of the 2-carboxy-6-hydroxyoctahydroindole moiety of aeruginosin, are reported.

Serendipitous crystallization and structure determination of cyanase (CynS) from Serratia proteamaculans

The cyanate hydratase CynS from S. proteamaculans was crystallized serendipitously during screens for a eukaryotic complex. Here, its crystal structure is described at 2.1 Å resolution.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of a type II NADH:quinone oxidoreductase from the human pathogen Staphylococcus aureus

The membrane protein NDH-II from the human pathogen S. aureus was produced in E. coli, purified and crystallized. The crystals belonged to the orthorhombic space group P2₁2₁2₁ and diffracted to 3.32 Å resolution; the structure was determined by molecular replacement.