 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](hv5289fig2mag.jpg)
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Figure 2
Activity assays validating functional PfOPRTase constructs. (a) Select PfOPRTase constructs can support the growth of OPRTase-deficient E. coli. Several variants of PfOPRTase-coding sequences [full length, PfOPRTase(51–218), PfOPRTase(73–218), PfOPRTase(Δ37–58) and PfOPRTase(Δ179–197)] in plasmid pET-28a were transformed into E. coli JW3617 cells. The cells were grown in M9 minimal medium (left half of the plate) or M9 medium supplemented with uracil (right half of the plate). Full-length PfOPRTase and partially deleted PfOPRTase (Δ37–58) successfully rescued E. coli JW3617 cells from OPRTase deficiency, but not the others. (b) Time-dependent reduction in absorbance as orotic acid is converted to orotidylate (OMP) by functional PfOPRTase. The change in the absorbance by orotic acid (at 296 nm) is shown in the presence of PfOPRTase (black), bovine serum albumin (green) or no added enzyme (orange). |
![[Figure 2]](hv5289fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
