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Figure 6
Flow diagram to cryoprotect a crystal. There are two major requirements for a good cryoprotectant solution: (i) it must be vitrified when flash-cooled (left side of the flowchart) and (ii) the crystal must tolerate the cryoprotecting treatment and diffract well when treated with the cryoprotectant solution (right side). Y, yes; N, no. Good technique is also required. Also, a test of crystal diffraction at room temperature is sometimes warranted as noted by the box at the upper right and the circle-enclosed RT symbols.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 71| Part 6| June 2015| Pages 622-642
https://doi.org/10.1107/S2053230X15008304
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