 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](tt5068fig2mag.jpg)
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Figure 2
(a) 12% SDS–PAGE gel of expressed S. aureus NAD synthetase. Lane M, molecular-weight markers (labelled in kDa); lane 1, Ni2+-affinity purified protein; lane 2, purified protein after size-exclusion chromatography. (b) Gel-filtration profile of S. aureus NAD synthetase from a Superdex S200 HR 10/300 column. The total volume of the column is 25 ml, while the void volume of the column is 8.17 ml. The protein eluted at 14.9 ml, which is consistent with a dimeric association in solution. |
![[Figure 2]](tt5068fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
