Figure 1
Human SapA crystallization and mounting. (a) SapA crystalline needle cluster grown by sitting-drop vapour diffusion against a reservoir consisting of 0.1 M sodium acetate pH 4.8, 0.2 M lithium sulfate, 25%(w/v) PEG 4000. A single needle (10 × 150 µm) is shown mounted in a nylon loop. (b) Details of crystal packing, showing views of the unit cell along the b (left) and a (right) axes. Two protein molecules are present per asymmetric unit (grey). The crystals contain 33.2% solvent, with a Matthews coefficient of 1.84 Å3 Da−1. |