Figure 2
X-ray crystal structure of SapA at lysosomal pH. (a) Monomeric SapA is shown in three orientations. Disulfide bonds are shown as yellow spheres. (b) The molecular surface is shown coloured by electrostatic potential at the solvent-accessible surface from red (negative, −3.0 kT e−1) to blue (positive, +3.0 kT e−1). Electrostatic potential was calculated using a pH value of 4.8 when assigning side-chain protonation. |