view article
Figure 1
Purification of recombinant AtaPT11–424. (a) Anion-exchange chromatography using a RESOURCE Q column. (b) Size-exclusion chromatography using a Superdex 200 10/300 HiLoad gel-filtration column. (c) SDS–PAGE analysis of AtaPT. The concentration of the gel is 12%(w/v). Lane 1, protein molecular-weight marker (Fermentas; labelled in kDa); lane 2, protein after GST affinity chromatography; lane 3, protein after anion-exchange chromatography; lane 4, protein after size-exclusion chromatography.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 71| Part 7| July 2015| Pages 889-894
https://doi.org/10.1107/S2053230X15008882
Follow Acta Cryst. F
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS