Figure 2
Selection of crystalline batch suspensions using SHG imaging microscopy. Rhodopsin batch crystallization trials [precipitant: 0.15 M DL-malic acid pH 7.0, 20%(w/v) PEG 3350] from the vials in Fig. 1(b) were tested for crystallinity by SHG imaging at 350 mW laser power (S1–S3) and for UV fluorescence by UV-TPEF (U1–U3). The scale bars represent 150 µm. The inset in the red box represents an enlargement of the typical grain-shaped micrometre-sized crystals (scale bar 50 µm) used in serial femtosecond crystallography. |