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Figure 8
(a) Fluorescence anisotropy assays using ctMex67ΔN–Mtr2. Data were fitted to the standard quadratic binding equation to obtain Kd values. All four RNA oligonucleotides tested were bound, albeit with different affinities. Similar to scMex67–Mtr2, ctMex67ΔN–Mtr2 bound polyA15 and polyG15 more tightly than polyU15 and polyG15. (b) Deletion of either the RRM or NTF2L–Mtr2 domains reduced the affinity of ctMex67 by >15-fold. On the other hand, deletion of the UBA domain from ctMex67ΔN–Mtr2 did not reduce the affinity towards polyA15 RNA significantly. Curves reproduced from (a) are shown as dotted lines without data points.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 71| Part 7| July 2015| Pages 876-888
https://doi.org/10.1107/S2053230X15008766
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