Figure 1
Stereo image of the cisplatin coordination to His15. A platinum ion is bound to both the Nδ and N∊ sides of the imidazole ring with ligands (two ammines and one Cl atom at Ptδ, and one chlorine, one ammine and the Arg14 side chain at Pt∊). Electron-density maps: 2Fo − Fc (in blue at 1.0 r.m.s.), Fo − Fc (in green at 5.0σ) and anomalous difference Fourier map (in orange at 3.0σ) of the final model. The two platinum ions are depicted in purple, the Cl atoms in brown, the N atoms in blue, O atoms in red and H atoms in charcoal grey. The orientation of each ammine's three H atoms and of the Arg14 side-chain H atoms at 1.7 Å diffraction resolution are obviously approximate (the Arg14 side-chain nitrogen closest to the Pt∊ in particular would present a lone pair of electrons to the metal atom). This figure was prepared using CCP4mg (McNicholas et al., 2011). |