Crystal structure of human GDF11

The structure of human growth differentiation factor 11 (GDF11) determined to a resolution of 1.50 Å reveals subtle conformational differences when compared with the previously determined structure of human myostatin (GDF8) while maintaining a highly similar quaternary architecture.

Crystal structure of maize serine racemase with pyridoxal 5′-phosphate

This is the first report of the crystal structure of a plant serine racemase.

Purification, crystallization and X-ray crystallographic analysis of a putative exopolyphosphatase from Zymomonas mobilis

A putative exopolyphosphatase (PPX) from Z. mobilis (ZmPPX) was expressed, purified and crystallized. PPX enzymes regulate intracellular inorganic polyphosphate levels in microbial cells in response to external stresses. N-terminal truncation of ZmPPX based on primary-sequence analysis resulted in an improvement in diffraction from 3.3 to 1.8 Å resolution.

Crystal structure of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase from the ESKAPE pathogen Acinetobacter baumannii

The crystal structure of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, a component of the shikimate pathway, was determined during its evaluation as a target for new antimicrobials effective against multidrug-resistant, extensively drug-resistant and pan-drug-resistant A. baumannii. This enzyme is essential for the growth and survival of this clinically important pathogen during host infection.

The use of noncrystallographic symmetry averaging to solve structures from data affected by perfect hemihedral twinning

Molecular replacement and noncrystallographic symmetry averaging were used to detwin a data set affected by perfect hemihedral twinning. The noncrystallographic symmetry averaging of the electron-density map corrected errors in the detwinning introduced by the differences between the molecular-replacement model and the crystallized structure.

Structure of a truncated form of leucine zipper II of JIP3 reveals an unexpected antiparallel coiled-coil arrangement

The crystal structure of an N-terminally truncated form of leucine zipper II (LZII) of JIP3 alone shows an unexpected antiparallel arrangement. This study draws attention to the fact that LZII of JIP3/JIP4 is a versatile structural motif, modifications of which can impact partner recognition and thus biological functions.

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of recombinant human C1ORF123 protein

Recombinant C1ORF123 protein from H. sapiens was overexpressed, purified to homogeneity and crystallized. Diffraction data were collected for X-ray crystallographic analysis.

Crystal structure of FhuD at 1.6 Å resolution: a ferrichrome-binding protein from the animal and human pathogen Staphylococcus pseudintermedius

The crystal structure of the FhuD protein from S. pseudintermedius was determined at 1.6 Å resolution. The structure displays a canonical class III solute-binding protein fold in a closed conformation, revealing a ligand-binding site suitable for the accommodation of siderophore ligands, here occupied by a polyethylene glycol molecule.

Neutron diffraction analysis of Pseudomonas aeruginosa peptidyl-tRNA hydrolase 1

The crystallization of perdeuterated P. aeruginosa peptidyl-tRNA hydrolase 1 and initial neutron diffraction data collection are reported.

Structural investigation of the thymidine phosphorylase from Salmonella typhimurium in the unliganded state and its complexes with thymidine and uridine

Structures of thymidine phosphorylase from S. typhimurium were determined in the unliganded state and in complexes with thymidine and uridine. The structural origins of the substrate specificity were found.

Purification, crystallization and initial crystallographic analysis of the α-catenin homologue HMP-1 from Caenorhabditis elegans

The purification, crystallization and initial X-ray crystallographic studies of the N-terminal and M domains of the α-catenin homologue HMP-1 from C. elegans are described.

Crystallization and X-ray diffraction analysis of the CH domain of the cotton kinesin GhKCH2

The cloning, expression, purification and crystallization of the CH domain of the plant-specific kinesin GhKCH2 is reported.

Crystal structure of recombinant tyrosinase-binding protein MtaL at 1.35 Å resolution

The crystal structure of MtaL shows that conformational changes are needed for binding to mushroom tyrosinase and reveals a putative carbohydrate-binding site that may be associated with glycoreceptor activity.

Re-refinement of 4xan: hen egg-white lysozyme with carboplatin in sodium bromide solution

An addendum is published to Tanley et al. [(2014), Acta Cryst. F70, 1135–1142].

Re-refinement of 4g4a: room-temperature X-ray diffraction study of cisplatin and its binding to His15 of HEWL after 14 months chemical exposure in the presence of DMSO

An addendum is published to Tanley et al. [(2012), Acta Cryst. F68, 1300–1306].

Applications of the second virial coefficient: protein crystallization and solubility. Corrigendum

The article by Wilson & DeLucas [(2014). Acta Cryst. F70, 543–554] is corrected.