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Figure 4
(a) A comparison of the background scatter of different plate types. The data are counts per pixel averaged over a constant-resolution ring against solid angle and are normalized to transmittance for each type and result from an exposure of ∼1.2 × 1011 photons at 12 800 eV in a beam size of approximately 10 × 10 µm. Plate types shown are Greiner CrystalQuickX, MiTeGen In Situ-1, a TPFS formed from a TTPLabtech viewdrop III cover (UV layer removed) (these three plate types are each sealed with HDClear tape) and finally an in-house-constructed TPFS formed from two layers of 13 µm ZeonorFilm with a 10 µm thick adhesive spacer. (b) Background scatter comparison using the same methodology as in (a) of the 13 µm ZeonorFilm TPFS formed with either a 100 µm spacer or a 10 µm spacer and contrasting measurements taken on and off a water drop. Air-scatter data for comparison give an approximation to ideal loop mounting (less any sample contribution).

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 72| Part 4| April 2016| Pages 313-319
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