Crystal structure of histone-like protein from Streptococcus mutans refined to 1.9 Å resolution

Streptococcal histone-like proteins (HLPs) are essential for bacterial survival. Here, the structure of S. mutans HLP was determined at 1.9 Å resolution.

Cloning, expression, purification and crystallization of Schizosaccharomyces pombe Set7, a putative histone methyltransferase

The methods used to clone, express, purify, crystallize and determine the phase of a full-length putative histone methyltransferase from yeast are described.

Crystal structure of the substrate-recognition domain of the Shigella E3 ligase IpaH9.8

Crystal structures of a leucine-rich repeat domain of IpaH9.8 from S. flexneri were determined in two crystal forms: P2₁2₁2₁ and C222₁.

Binding of Gd³⁺ to the neuronal signalling protein calexcitin identifies an exchangeable Ca²⁺-binding site

Calexcitin is a two-domain, neuron-specific signalling protein which possesses four EF-hand motifs, of which only the first three are functional metal-binding sites. Recombinant calexcitin from L. pealei has been crystallized with an equimolar concentration of Gd³⁺ in the presence of trace Ca²⁺, and it was demonstrated that it is the Ca²⁺ ion bound to the N-terminal EF-hand which becomes replaced by the exogenous heavy-metal ions.

Isolation, crystallization and crystal structure determination of bovine kidney Na⁺,K⁺-ATPase

The isolation of bovine kidney Na⁺,K⁺-ATPase is presented, allowing the successful crystallization and crystal structure determination of an ouabain-bound E2–BeF₃⁻ complex.

Crystal structure of a putative exo-β-1,3-galactanase from Bifidobacterium bifidum S17

The production, crystallization and X-ray structure of a putative exo-β-1,3-galactanase from B. bifidum S17 (BbGal43A) are reported. BbGal43A is a putative single-domain enzyme from glycoside hydrolase family 43, which possibly acts as a dimer.

Purification, crystallization and X-ray diffraction analysis of the DNA-binding domain of human heat-shock factor 2

The DNA-binding domain (DBD; residues 7–112) of human heat-shock factor 2 was purified and crystallized. An X-ray diffraction data set was collected to 1.32 Å resolution, which is suitable for the structure determination of the first mammalian heat-shock factor DBD at atomic resolution.

Crystal structures of Leishmania mexicana arginase complexed with α,α-disubstituted boronic amino-acid inhibitors

Crystal structures of L. mexicana arginase complexed with two α,α-disubstituted boronic amino-acid inhibitors were determined to resolutions of 1.28 and 1.65 Å, respectively.

A novel microseeding method for the crystallization of membrane proteins in lipidic cubic phase

A method of making crystal seed stocks for random microseed matrix screening in LCP is introduced with a practical example that shows that the number of crystallization hits can be increased. Furthermore, an enhancement of the method that generates seed gradients is demonstrated.

Applications of thin-film sandwich crystallization platforms

Crystallization via sandwiches of thin polymer films is presented and discussed.

Crystal structure of a tankyrase 1–telomere repeat factor 1 complex

Tankyrase 1 (TNKS1) regulates the activity of telomere repeat factor 1 (TRF1) through PARylation. Here, the crystal structure of a complex between TRF1 (residues 1–55) and the second and third ankyrin-repeat clusters of TNKS1 is presented.

Crystal structure of CotA laccase complexed with 2,2-azinobis-(3-ethylbenzo­thiazoline-6-sulfonate) at a novel binding site

The crystal structure of CotA complexed with 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) in a hole motif has been solved; this novel binding site could be a potential structure-based target for protein engineering of CotA laccase.

Errors in Crystal structure of HINT from Helicobacter pylori

The article, Crystal structure of HINT from Helicobacter pylori by Tarique et al. [(2016) Acta Cryst. F72, 42–48], and HINT nomenclature are discussed.

Response to Errors in Crystal structure of HINT from Helicobacter pylori

A response to the article by Maize [(2016), Acta Cryst. F72, 336-337].