 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](tb5099fig1mag.jpg)
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Figure 1
Purification of the human RBM7 RRM domain. (a) A 15% SDS–PAGE stained with Coomassie Brilliant Blue showing the progress of protein purification. His-SUMO-RBM7 1–91 was first isolated by Ni–NTA chromatography before removal of the His-SUMO tag by Ulp protease cleavage and subsequent separation by Ni–NTA chromatography. Lane M contains molecular-mass marker (labelled in kDa). (b) RBM7 1–91 elutes at 14.2 ml on a Superdex 75 10/300 column, corresponding to a molecular weight of ∼12 kDa. The void volume (V0) and elution volumes of standard proteins are shown above the chromatogram (A280, blue; A260, red). |
![[Figure 1]](tb5099fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
