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Figure 3
2Fo − Fc OMIT maps obtained after ten cycles of additional OMIT refinement of the two models, leaving out the mutated side chains and the ligand glycolic acid (GOA). (a) Ma18, near E23G (contour level 1σ). (b) Ma18, near A70T (contour level 0.8σ). (c) Ma18, near S96F (contour level 0.8σ). (d) Ma18, near A178V (contour level 0.8σ). (e) Ma21, near Q65L (contour level 0.8σ). (f) Ma21, near the bound glycolic acid (GOA) (contour level 1σ). The refined models are shown in pink and the A-TIM reference model (PDB entry 2vek) is shown in green. |