Structural basis for DNA recognition by the transcription regulator MetR

The crystal structure of the DNA-binding domain (DBD) of the transcription regulator MetR from E. coli was solved at 2.16 Å resolution. The DNA-recognition mechanism of the DBD of MetR was explored by macromolecular docking simulations.

Crystal structure of the TK2203 protein from Thermococcus kodakarensis, a putative extradiol dioxygenase

The crystal structure of the TK2203 protein was determined at 1.41 Å resolution.

Cyclization strategies of meditopes: affinity and diffraction studies of meditope–Fab complexes

An overview of cyclization strategies of a Fab-binding peptide to maximize affinity.

Putative thioredoxin Trx1 from Thermosipho africanus strain TCF52B: expression, purification and structural determination using S-SAD

Crystallographic analysis of the putative thioredoxin Trx1 from T. africanus strain TCF52B, which has low sequence identity to its closest homologues, was completed using sulfur single-wavelength anomalous dispersion analysis.

Crystal structure of glutamate-1-semi­aldehyde-2,1-aminomutase from Arabidopsis thaliana

A structural study of A. thaliana glutamate-1-semialdehyde-2,1-aminomutase (GSAM) has revealed asymmetry in cofactor binding as well as in the gating-loop orientation, which supports the previously proposed negative cooperativity between monomers of GSAM.

The SecB-like chaperone Rv1957 from Mycobacterium tuberculosis: crystallization and X-ray crystallographic analysis

Rv1957 from M. tuberculosis is a SecB-like protein that functions in the higBA toxin–antitoxin system. Here, the crystallization and X-ray crystallographic analysis of Rv1957 are described.

Crystal structure of a chimaeric bacterial glutamate de­hydrogenase

The structure of the first chimaeric glutamate dehydrogenase has been determined at 2.7 Å resolution. This active enzyme was designed from the NADP⁺-binding domain of the E. coli enzyme and the substrate-binding domain of the enzyme from C. symbiosum.

Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine

A solute-binding protein from Pfam13407 was found to be specific for D-glucosamine/D-galactosamine by differential scanning fluorimetry and X-ray crystallography.

Structure of the I-SceI nuclease complexed with its dsDNA target and three catalytic metal ions

The structure of the homing endonuclease I-SceI from S. cerevisiae has been determined in complex with its target DNA and catalytic cations, revealing a two-metal-ion mechanism.

Plant-specific DUF1110 protein from Oryza sativa: expression, purification and crystallization

The Os01T0156300 protein, a plant-specific DUF1110 protein from O. sativa, was expressed, purified and crystallized. An X-ray diffraction data set was collected from a native crystal to 1.84 Å resolution.

The VapBC1 toxin–antitoxin complex from Mycobacterium tuberculosis: purification, crystallization and X-ray diffraction analysis

Bacterial VapBC modules belong to the type II toxin–antitoxin systems, which function in many important biological processes, such as the stringent response and drug tolerance. The toxin VapC1 and the cognate antitoxin VapB1 from M. tuberculosis were separately cloned and co-expressed in E. coli BL21(DE3) cells. Diffraction-quality crystals were obtained by using commercial sparse-matrix screen solutions as additives.

Crystal structures of two monomeric triosephosphate isomerase variants identified via a directed-evolution protocol selecting for L-arabinose isomerase activity

Crystal structures are described of two monomeric triosephosphate isomerase (TIM) variants (Ma18, with four point mutations, and Ma21, with one point mutation) that were identified via a directed-evolution protocol (starting from A-TIM) and that have enhanced in vivo L-arabinose isomerase activity. The two structures show that the point mutations cause only minor structural changes, and for Ma21 the small structural changes near Asn11 of loop 1 rationalize its different binding properties.

High-resolution NMR structures of the domains of Saccharomyces cerevisiae Tho1

In this study, high-resolution structures of both the N-terminal DNA-binding SAP domain and the C-terminal RNA-binding domain of S. cerevisiae Tho1 have been determined.