Figure 1
Purification, crystallization and X-ray diffraction of the N-PAS domain. (a) SDS–PAGE of the eluate from the HiLoad 26/60 Superdex 200 size-exclusion column. The lane on the right contains molecular-mass markers (labelled in kDa). The target protein is located at ∼17 kDa. (b) The crystal of the N-PAS domain used for the collection of X-ray data. (c) Representative diffraction image from a crystal of the hEAG N-PAS domain. |