Crystal structure of the PAS domain of the hEAG potassium channel

The structure of the hEAG PAS domain is presented and compared with similar segments of human and insect ion channels.

Envelope protein VP24 from White spot syndrome virus: expression, purification and crystallization

VP24 is one of the major envelope proteins of White spot syndrome virus. In order to facilitate purification, crystallization and structure determination, the predicted N-terminal transmembrane region of approximately 26 amino acids was truncated from VP24 and several mutants were prepared to increase the ratio of methionine for subsequent data collection using the SAD method.

Malonate in the nucleotide-binding site traps human AKAP18γ/δ in a novel conformational state

AKAP18γ/δ is recognized for its involvement in modulating cardiac Ca²⁺ release and re-uptake and is a potential drug target in cardiovascular disease. The atomic resolution crystal structure of the central domain of AKAP18γ/δ complexed with malonate presents an optimal template for structure-guided drug design.

Structure of the human DNA-repair protein RAD52 containing surface mutations

The structure of the N-terminal half of the human RAD52 protein containing three alanine substitutions was solved at 2.4 Å resolution. The structure revealed a previously unobserved interaction between RAD52 rings in which the C-terminal end of one of the monomers in the RAD52 ring associates with the positively charged groove of a symmetry-related RAD52 ring in the crystal lattice.

Crystallization and X-ray diffraction analysis of an L-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii and a D-xylonate dehydratase from Caulobacter crescentus

L-Arabinonate dehydratase and D-xylonate dehydratase from the IlvD/EDD family were crystallized by the vapour-diffusion method. Diffraction data sets were collected to resolutions of 2.40 and 2.66 Å from crystals of L-arabinonate dehydratase and D-xylonate dehydratase, respectively.

Structure of the lutein-binding domain of human StARD3 at 1.74 Å resolution and model of a complex with lutein

The structure of a START-domain protein known to bind lutein in the human retina is reported to an improved resolution limit. Rigid-body docking demonstrates that at least a portion of lutein must protrude from the large tunnel-like cavity characteristic of this helix-grip protein and suggests a mechanism for lutein binding specificity.

Structural analysis of a function-associated loop mutant of the substrate-recognition domain of Fbs1 ubiquitin ligase

The crystal structure of the substrate-recognition domain of an Fbs1 mutant was determined at a resolution of 2.3 Å. Comparison of the wild-type and mutant Fbs1 structures provides insight into the structural features of this carbohydrate-binding site.

Crystal structures of a subunit of the formyl­glycinamide ribonucleotide amidotransferase, PurS, from Thermus thermophilus, Sulfolobus tokodaii and Methanocaldococcus jannaschii

Crystal structures of a subunit of the formylglycinamide ribonucleotide amidotransferase, PurS, from T. thermophilus, S. tokodaii and M. jannaschii are determined and their structural characteristics are analyzed.

Crystal structure of the antigen-binding fragment of a monoclonal antibody specific for the multidrug-resistance-linked ABC transporter human P-glycoprotein

The monoclonal antibody UIC2 binds specifically to the extracellular region of the human P-glycoprotein in a conformationally sensitive manner. The crystal structure of the Fab fragment of UIC2 is reported.

Crystal structure of Rv3899c^184–410, a hypothetical protein from Mycobacterium tuberculosis

The crystal structure of Rv3899c^184–410 was found to contain a compact helix bundle and an α/β/α sandwich folding domain.

Characterization of the NTPR and BD1 interacting domains of the human PICH–BEND3 complex

The interaction between PICH and BEND3 is dissected and the domains involved in their interaction have been crystallized.

Crystal structure of AibC, a reductase involved in alternative de novo isovaleryl coenzyme A biosynthesis in Myxococcus xanthus

The crystal structure of AibC, the terminal reductase in myxococcal de novo isovaleryl coenzyme A biosynthesis, has been determined at 2.55 Å resolution.