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Figure 1
Expression and purification of VP24. Lane 1, total cell lysate; lane 2, supernatant from the cell lysate; lane 3, pellet from the cell lysate; lane 4, flowthrough of the supernatant from an NTA affinity column; lanes 5 and 6, 20 and 50 mM imidazole washes from the NTA affinity column, respectively; lane 7, 500 mM imidazole eluate from the NTA affinity column; lane 8, molecular-weight markers (labelled in kDa). |