Figure 2
Representative native crystals of FapF106–430. Crystals formed over 5–7 d in the reservoir condition 100 mM sodium citrate, 20–30%(w/v) PEG 400, 100 mM NaCl. Purified FapF106–430 in 20 mM Tris–HCl pH 8.0, 150 mM NaCl, 0.5% C8E4 was concentrated to 10 mg ml−1. Scale bar 500 µm. |