1.45 Å resolution structure of SRPN18 from the malaria vector Anopheles gambiae

The structure of the A. gambiae serine proteinase inhibitor (serpin) SRPN18 was determined, revealing an unusually short and constricted reactive-center loop.

Crystal structure of the toxin Msmeg_6760, the structural homolog of Mycobacterium tuberculosis Rv2035, a novel type II toxin involved in the hypoxic response

The crystal structure of Msmeg_6760 reveals a novel fold for a toxin that harbors a large central cavity that is likely to bind a large hydrophobic compound.

The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae

A cellulase from K. pneumoniae was cloned, expressed, purified and shown to be an endoglucanase. Its structure was determined at 1.76 Å resolution and comparisons with homologous enzymes provide information on substrate specificity.

Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights

The expression, purification, crystallization, X-ray powder diffraction data collection and preliminary analysis of protease 3C from coxsackievirus B3 are reported.

The crystal structure of di­hydrodipicolinate reductase from the human-pathogenic bacterium Bartonella henselae strain Houston-1 at 2.3 Å resolution

The cloning, expression, purification, crystallization and X-ray diffraction analysis of dihydrodipicolinate reductase from the human-pathogenic bacterium B. henselae, the causative bacterium of cat-scratch disease, are reported.

Purification, crystallization and characterization of the Pseudomonas outer membrane protein FapF, a functional amyloid transporter

FapF from Pseudomonas facilitates the secretion of the amyloid-forming polypeptide FapC across the bacterial outer membrane. A protocol for forming reproducible well diffracting crystals is described in which the switch to tetraethylene glycol monooctyl ether detergent during purification proved to be critical and represents a handy tip for β-barrel protein crystallography.

Overcoming a hemihedral twinning problem in tetra­hydrofolate-dependent O-de­methylase crystals by the microseeding method

A hemihedral twinning problem was found in crystals of a tetrahydrofolate-dependent O-demethylase, LigM, from Sphingobium sp. SYK-6. The problem was overcome using microseeding.

Characterization and 1.57 Å resolution structure of the key fire blight phosphatase AmsI from Erwinia amylovora

The high-resolution (1.57 Å) structure and kinetic data of the key fire blight phosphatase AmsI are reported.

Protein production, crystallization and preliminary crystallographic analysis of the four N-terminal immunoglobulin domains of Down syndrome cell adhesion molecule 1. Corrigendum

A corrigendum is published to the article by Chen et al. [(2015), Acta Cryst. F71, 775–778].