Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights

Fili, S.; Valmas, A.; Christopoulou, M.; Spiliopoulou, M.; Nikolopoulos, N.; Lichière, J.; Logotheti, S.; Karavassili, F.; Rosmaraki, E.; Fitch, A.; Wright, J.; Beckers, D.; Degen, T.; Nenert, G.; Hilgenfeld, R.; Papageorgiou, N.; Canard, B.; Coutard, B.; Margiolaki, I.

doi:10.1107/S2053230X16018513

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Analysis of the expression and purification of CVB3 3C^pro. (a) Analysis of 3C protease expression in E. coli on a Coomassie Blue-stained SDS–PAGE gel. Lane 1, sample from the supernatant after overnight expression. Lane M, molecular-weight marker (labelled in kDa). (b) Analysis of the purification of CVB3 3C^pro expressed in E. coli on a Coomassie Blue-stained SDS–PAGE gel. Lane M, molecular-weight marker (labelled in kDa); lanes marked with an asterisk are the selected fractions containing CVB3 3C^pro after size-exclusion chromatography.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 72| Part 12| December 2016| Pages 877-884

https://doi.org/10.1107/S2053230X16018513

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