Crystal structure of the type IV secretion system component CagX from Helicobacter pylori

Zhang, J.; Fan, F.; Zhao, Y.; Sun, L.; Liu, Y.; Keegan, R.M.; Isupov, M.N.; Wu, Y.

doi:10.1107/S2053230X17001376

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 1
15% SDS–PAGE analysis of purified CagXct stained with Coomassie Brilliant Blue. Lane A, non-induced expression strains. Lane a, induced expression strains. Lane B, pellet fractions. Lane b, supernatant fractions. Lane 1, eluted with 500 mM imidazole; the band containing CagXct with a Trx tag is around 35 kDa. Lane 2, after cleavage by TEV; the band containing CagXct is under 14.4 kDa. Lane 3, flowthrough of the second nickel column. Lane 4, the sample before size-exclusion chromatography, showing high purity. Lane M contains molecular-mass marker (labelled in kDa).

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 73| Part 3| March 2017| Pages 167-173

https://doi.org/10.1107/S2053230X17001376

Open

access

Follow Acta Cryst. F

Search IUCr Journals		doi		Advanced search
Author		volume	page