Crystal structure of full-length Zika virus NS5 protein reveals a conformation similar to Japanese encephalitis virus NS5

The recent epidemic of Zika virus (ZIKV) has created a major global health concern. Here, the crystal structure of the full-length ZIKV NS5 protein at 3.05 Å resolution is presented to facilitate the structure-based design of antiviral agents against ZIKV.

Crystallization of FcpA from Leptospira, a novel flagellar protein that is essential for pathogenesis

FcpA from Leptospira interrogans, which is essential for pathogenesis, was crystallized but proved to be recalcitrant to X-ray diffraction studies. The orthologue from the saprophyte L. biflexa allowed three different crystal forms to be obtained that were suitable for crystallographic studies.

The crystal structure of mouse LC3B in complex with the FYCO1 LIR reveals the importance of the flanking region of the LIR motif

FYCO1 mediates microtubule plus-end-directed autophagosome transport by interacting with LC3. Here, the crystal structure of mouse LC3B in complex with the FYCO1 LC3-interacting region (LIR) motif peptide was determined.

Proteolysis of truncated hemolysin A yields a stable dimerization interface

Structural analysis of two variant forms of truncated hemolysin A reveals additional proteolysis of the C-terminal 31 amino acids under high-salt crystallization conditions. This structural plasticity may support the implementation of stably exposed, on-edge β-strands during template-assisted folding.

Similarities in the structure of the transcriptional repressor AmtR in two different space groups suggest a model for the interaction with GlnK

The structure of ligand-free and DNA-unbound AmtR, a TetR-type global nitrogen regulator from C. glutamicum, was solved at 2.1 Å resolution in space group P2₁2₁2 with six molecules in the asymmetric unit. A structure comparison with the previously solved structure of AmtR in a trigonal crystal system suggests a 6:6 stoichiometric association of three AmtR dimers with two trimeric GlnK complexes that prevents AmtR from binding to DNA and induces gene transcription.

The hyperthermophilic cysta­thionine γ-synthase from the aerobic crenarchaeon Sulfolobus tokodaii: expression, purification, crystallization and structural insights

Cystathionine γ-synthase, a pyridoxal 5′-phosphate (PLP)-dependent enzyme from the thermoacidophilic archaeon S. tokodaii, was crystallized under three conditions. The structural data suggested that the orientations of the PLP cofactor and the active-site residues differed among the three forms, which might be key to the activity of the enzyme under extreme conditions.

New molecular packing in a crystal of pseudoazurin from Alcaligenes faecalis: a double-helical arrangement of blue copper

A new molecular packing of pseudoazurin from A. faecalis shows a right-handed double helix composed of blue copper sites.

Crystal structure of the type IV secretion system component CagX from Helicobacter pylori

The structure of the C-terminal domain of CagX is presented and structural comparisons with TraO, its homologue from another bacterial T4S system, reveal distinct and conserved features.