 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](hv5345fig1mag.jpg)
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Figure 1
Purification of the 7β-HSDH protein. (a, b) Analytic size-exclusion column chromatography of the purified 7β-HSDH protein. The absorbance of the eluate was monitored at 280 nm. The protein markers used to produce the the standard curve in (b) were purchased from GE Healthcare and were used according to the manufacturer's protocol. (c) Coomassie-stained 12% SDS–PAGE showing the purity of 7β-HSDH. Lane 1, molecular-weight markers; lane 2, 5 µg 7β-HSDH; lane 3, 10 µg 7β-HSDH; lane 4, 20 µg 7β-HSDH. (d) LC-MS shows the calculated molecular weight of the purified 7β-HSDH protein. (e) ITC profiles for the titration of 7β-HSDH with NADP+. |
![[Figure 1]](hv5345fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
