Structure of NADP⁺-bound 7β-hydroxysteroid de­hydrogenase reveals two cofactor-binding modes

The structure of a binary complex of NADP⁺ with 7β-hydroxysteroid dehydrogenase reveals two modes of cofactor binding.

Increasing the soluble expression and crystallization of the Escherichia coli quorum-sensing protein LsrK

LsrK from E. coli phosphorylates imported AI-2 molecules and triggers quorum-sensing signalling. The protein was expressed by applying heat and osmotic shock before IPTG induction, and a crystal of LsrK diffracted to 2.9 Å resolution.

Crystal structure of d(CCGGGGTACCCCGG)₂ at 1.4 Å resolution

The structure of the tetradecamer sequence d(CCGGGGTACCCCGG)₂ is reported here as an A-DNA duplex at 1.4 Å resolution.

Structural insights into the mechanism of the drastic changes in enzymatic activity of the cytochrome P450 vitamin D₃ hydroxylase (CYP107BR1) caused by a mutation distant from the active site

The structures of inactive mutants (F106V and L348M) of the cytochrome P450 vitamin D₃ hydroxylase were determined at 1.95 and 2.7 Å resolution, respectively.

Crystal structure of the RNA 2′,3′-cyclic phosphodiesterase from Deinococcus radiodurans

The RNA 2′,3′-cyclic phosphodiesterase (CPDase) from D. radiodurans was crystallized and the crystals diffracted to 1.6 Å resolution. The structure, with an open active site, reveals an elegant mechanism for a CPDase enzyme, suggesting a conserved mechanism of catalysis.

Transcription factor Rv0081 from Mycobacterium tuberculosis: purification, crystallization and initial crystallographic analysis

The response regulator Rv0081 is an essential regulatory hub for promoting the long-term survival of M. tuberculosis. Here, the production, crystallization and initial X-ray crystallographic analysis of Rv0081 are reported.

Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis

The structures of four nanobodies raised against the periplasmic domain of PorM were solved, and their interaction with their target was analysed. Diffracting crystals of two PorM constructs in complex with their specific nanobodies were obtained.

Crystal structure of CD27 in complex with a neutralizing noncompeting antibody

The crystal structure of human CD27 was determined at a resolution of 1.8 Å, revealing the assembly of the cysteine-rich domains. The binding of the antibody at the edge of the receptor molecule is unusual for the tumor necrosis factor receptor superfamily.

Crystallization and biochemical characterization of an archaeal lectin from Methanococcus voltae A3

An archaeal lectin from Methanococcus voltae A3 has been cloned, expressed, purified and crystallized, and the crystals have been characterized using X-ray diffraction.