Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis

Duhoo, Y.; Roche, J.; Trinh, T.T.N.; Desmyter, A.; Gaubert, A.; Kellenberger, C.; Cambillau, C.; Roussel, A.; Leone, P.

doi:10.1107/S2053230X17005969

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Sequence alignment of the four nanobodies nb01, nb02, nb19 and nb130 raised against the periplasmic domain of PorM. Secondary-structure elements from the nb01 structure (molecule A) are displayed above the alignment. The CDR1, CDR2 and CDR3 sequences are boxed; the cysteine residues are denoted by asterisks. Kabat numbering is used (Kabat et al., 1991

). The sequence alignment was generated by DeepAlign (Wang et al., 2013

) on the basis of the superposition of the four nanobody structures (molecule A for nb01 and nb19) and processed by ESPript (Robert & Gouet, 2014

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 73| Part 5| May 2017| Pages 286-293

https://doi.org/10.1107/S2053230X17005969

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