Figure 1
Formation and characterization of Fab–NHBA complexes. (a) SDS–PAGE analysis under reducing (left) and nonreducing (right) conditions of purified NHBAp2 (lane 1), Fab 10C3 (lane 2), the 10C3–NHBA complex (lane 3), Fab 12E1 (lane 4) and the 12E1–NHBA complex (lane 5). (b) Size-exclusion chromatography elution profiles of NHBAp2 (grey), Fab 10C3 (green), the 10C3–NHBA complex (magenta), Fab 12E1 (cyan) and the 12E1–NHBA complex (blue). Each chromatogram refers to an independent run. |