Figure 1
Elution profile of MarRC.difficile from a Superdex 200 HiLoad 16/60 gel-filtration column on an ÄKTAexplorer FPLC system. The peak at 82 ml (the flow rate was 1 ml min−1) represents the MarRC.difficile dimer. The apparent molecular weight of the eluting species was calculated using standard protein markers (Gel Filtration LMW Calibration Kit, GE Healthcare). The right panel shows SDS–PAGE analysis of the collected fraction. |