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Figure 1
(a) Size-exclusion chromatography profile of CtPGT. The molecular size of the CtPGT protein was ∼42 kDa as calculated using protein standards. The graph represents logKd plotted against the log of the molecular weights of the protein standards (open circles) and CtPGT (filled circle). Kd was derived from the equation Kd = (Ve − Vo)/(Vt − Vo), where Ve, Vo and Vt represent the elution volume of each standard protein, the void volume and the total volume of the column, respectively. The standard proteins were β-amylase (200 kDa), bovine serum albumin (66 kDa), carbonic anhydrase (29 kDa) and cytochrome c (12.4 kDa). (b) SDS–PAGE gel of the corresponding peak elution fractions (27–34) shown with markers (labelled in kDa). (c) CtPGT protein crystals grown by the hanging-drop vapour-diffusion method. The scale bar represents 0.4 mm.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 73| Part 11| November 2017| Pages 629-634
https://doi.org/10.1107/S2053230X17015515
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