 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 3]](nw5071fig3mag.jpg)
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Figure 3
Competition analysis between APIM and T3 by pull-down assay. The left panel shows PCNA bound to T3 (PDB entry 3vkx; Punchihewa et al., 2012  ). The molecular surface of PCNA is shown in cyan and blue (IDCL) and T3 is shown in stick representation. Lane 1 contains PCNA only. Lane 2 contains GST-ZRANB3(861–1079), which includes the APIM peptide, incubated with PCNA as a control (CTL). Lanes 3 and 4 contain GST-ZRANB3(861–1079) incubated with PCNA in the presence of an equimolar amount and a tenfold molar excess of T3, respectively. The double dagger indicates the degradation products of GST-ZRANB3(861–1079) that supposedly retain the GST moiety but lack the C-terminal APIM region, and are independent of the interaction between GST-ZRANB3(861–1079) and PCNA. The relative band intensities of PCNA divided by the band intensities of GST-ZRANB3(861–1079) were normalized to those of CTL (lane 2), and the values obtained are presented as bar graphs with standard error (SE) bars (n = 3). Asterisks indicate statistically significant differences from the control (CTL) (Dunnett's test; *, p < 0.05; **, p < 0.01). |
![[Figure 3]](nw5071fig3.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
