Crystal structure of chorismate mutase from Burkholderia phymatum

The crystal structure of chorismate mutase from B. phymatum reveals the prototypical AroQγ topology, as observed in the homolog from M. tuberculosis.

Crystal structure of pyrimidine-nucleoside phosphorylase from Bacillus subtilis in complex with imidazole and sulfate

Pyrimidine-nucleoside phosphorylases participate in DNA/RNA synthesis and are essential in anticancer drug therapies. Here, the structure of pyrimidine-nucleoside phosphorylase from B. subtilis in complex with imidazole is reported at 1.88 Å resolution.

Bacteriophage N4 large terminase: expression, purification and X-ray crystallographic analysis

The putative large and small terminase genes of bacteriophage N4 that are essential for genome packaging were identified, cloned and expressed, and the proteins were purified. The purified wild-type large terminase exhibited basal and small terminase-stimulated ATPase activity and produced thin plate-like crystals under one condition.

Structural view of the helicase reveals that Zika virus uses a conserved mechanism for unwinding RNA

This article reports the crystal structure of the apo form and the AMPPNP- and Mn²⁺- bound forms of the essential helicase of ZIKV refined to 1.78 and 1.3 Å resolution, respectively.

Structure of proliferating cell nuclear antigen (PCNA) bound to an APIM peptide reveals the universality of PCNA interaction

The crystal structure of proliferating cell nuclear antigen (PCNA) bound to a peptide carrying an AlkB homologue 2 PCNA-interacting motif consensus sequence, RFLVK, was determined. The phenylalanine and leucine residues of the peptide, plus a preceding hydrophobic residue, are involved in interactions with PCNA, providing a structural basis for regulation of the PCNA interaction.

Crystal structure of RecR, a member of the RecFOR DNA-repair pathway, from Pseudomonas aeruginosa PAO1

The 2.2 Å resolution crystal structure of the homologous recombination protein RecR from P. aeruginosa PAO1 is reported. The crystal structure shows that dimeric P. aeruginosa RecR forms a ring-like tetramer architecture via crystal symmetry.

The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical characterization and crystallographic data

The putative compatible solute-binding protein ProX from Mycobacterium tuberculosis was found to bind polyphenols instead of betaine, choline or carnitine. X-ray diffraction data were collected to 2.10 Å resolution.

High-resolution crystal structure of Streptococcus agalactiae glyceraldehyde-3-phosphate de­hydrogenase

The crystal structure of glyceraldehyde-3-phosphate dehydrogenase from S. agalactiae is reported at 1.36 Å resolution.

The crystal structure of the drug target Mycobacterium tuberculosis me­thionyl-tRNA synthetase in complex with a catalytic intermediate

The crystal structure of methionyl-tRNA synthetase (MetRS) from Mycobacterium tuberculosis in complex with the catalytic intermediate methionyl adenylate at 2.6 Å resolution is described. Comparisons with other MetRSs, including human cytosolic MetRS, reveal substantial differences that could be of use in the development of new antituberculosis inhibitors.

Structural view of the 2A protease from human rhinovirus C15

The 2.6 Å resolution crystal structure of the 2A protease from human rhinovirus C15 (HRV-C15) reveals the presence of a conserved His–Asp–Cys catalytic triad and a Zn²⁺-binding site. Comparison with the structures of 2A proteases from other enteroviruses reveals that the substrate-binding cleft of the 2A protease from HRV-C15 shows a more open conformation and is presumably more accessible for substrate binding.

Crystallization of the rice immune receptor RGA5A_S with the rice blast fungus effector AVR1-CO39 prepared via mixture and tandem strategies

Complexes of the rice immune receptor RGA5A_S and the rice blast fungus effector AVR1-CO39 were prepared using mixing, by fusing the two proteins in one gene construct and by co-expression. Diffracting crystals were obtained using the first two methods under two different conditions.