Figure 4
(a) Stereoview of the structural overlay of ZRANB3 APIM (yellow) and PIPMs: p21 (PDB entry 1axc; silver), Pol-ι (PDB entry 2zvm; pale pink) and Pol-η (PDB entry 2zvk; pale cyan; Hishiki et al., 2009). Residues of ZRANB3 APIM are labelled. (b) Binding of the noncanonical PIPM of Pol-ι to PCNA. The structure of Pol-ι bound to PCNA is shown as a pale pink tube (PDB entry 2zvm). Residues of the `hydrophobic plug' (Ile449, Tyr452 and Leu453) are shown in stick representation and are labelled. Residues engaged in intramolecular contacts (Lys446 and Tyr451) are also shown. PCNA is shown as a surface representation and the colours correspond to those in the left panel of Fig. 2(c). |